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The mutants have been analyzed by inverse PCR, applying two oligonucleotides, OTn1 and OTn2, to amplify adjacent DNA fragment for the transposon insertion web site, followed by sequencing of the cloned product (see Materials and Methods
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mutants faulty in distinctive phases of biofilm development, many components that take part in the initiation of biofilm formation and progression to microcolonies and mature biofilm had been identified (9). Through the analysis in the phenotypes of different P. aeruginosa
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This really conserved pathway in Gram-negative bacteria is involved with the assembly of more than 30 adhesive organelles (fourteen). These organelles are mostly fimbrial constructions which will adopt distinct morphologies. Two most important lessons might be discerned. The thick and rigid pili (7 nm in diameter), such as the P or form one pili from E. coli
adhesins, related to the adhesins in other microorganisms (14). We confirmed that mutants devoid of a useful CupA are faulty during the development of biofilm, in a very method which is independent of the existence of style IV pili. Utilizing the accessible sequence from the PAO1 genome, we determined additional cup
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